38.3 Folate

38.3.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 28.8877868 1 <0.0001 ***
Folate 0.0716672 1 0.8 ns
Study 0.1542094 1 0.7 ns
GA_samp 20.3225866 1 <0.0001 ***
Folate:Study 0.0092019 1 0.9 ns
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 4.6343389 1 0.03 *
Folate 0.2677523 1 0.6 ns
Study 0.4571143 1 0.5 ns
GA_samp 315.8716375 1 <0.0001 ***
Folate:Study 0.5568254 1 0.5 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 22.5067527 1 <0.0001 ***
Folate 0.9603731 1 0.3 ns
Study 1.3118341 1 0.3 ns
GA_samp 94.5221604 1 <0.0001 ***
Folate:Study 1.0202298 1 0.3 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 Folate 1 Inf 3.490 3.490 0.06 ns
3 Study 1 Inf 2.492 2.492 0.1 ns
4 GA_samp 1 Inf 0.842 0.842 0.4 ns
2 Folate:Study 1 Inf 0.214 0.214 0.6 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 Folate 1 Inf 5.087 5.087 0.02 *
3 Study 1 Inf 4.830 4.830 0.03 *
4 GA_samp 1 Inf 0.376 0.376 0.5 ns
2 Folate:Study 1 Inf 0.326 0.326 0.6 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 Folate 1 Inf 3.720 3.720 0.05 ns
3 Study 1 Inf 11.156 11.156 0.0008 ***
4 GA_samp 1 Inf 0.399 0.399 0.5 ns
2 Folate:Study 1 Inf 0.707 0.707 0.4 ns

38.3.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 14.41 (5.73, 36.08) <0.0001
Folate 1.00 (1.00, 1.00) 0.29
StudySTOP 1.12 (0.81, 1.54) 0.49
GA_samp 1.14 (1.07, 1.20) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.68 (1.00, 2.79) 0.05
Folate 1.00 (1.00, 1.00) 0.61
StudySTOP 1.19 (0.99, 1.44) 0.06
GA_samp 1.33 (1.29, 1.38) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.16 (0.08, 0.32) <0.0001
Folate 1.00 (1.00, 1.00) 0.94
StudySTOP 0.93 (0.73, 1.19) 0.56
GA_samp 1.22 (1.17, 1.27) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 20.29 (5.34, 77.09) <0.0001
Folate 1.00 (1.00, 1.00) 0.12
StudySTOP 0.64 (0.40, 1.02) 0.06
GA_samp 1.04 (0.96, 1.13) 0.36
HOMA-IR
Var Effect P
(Intercept) 6.54 (1.57, 27.17) 0.010
Folate 1.00 (0.99, 1.00) 0.05
StudySTOP 0.50 (0.31, 0.83) 0.007
GA_samp 1.03 (0.94, 1.12) 0.54
Glucose (mmol/L)
Var Effect P
(Intercept) 7.10 (5.46, 9.23) <0.0001
Folate 1.00 (1.00, 1.00) 0.02
StudySTOP 0.79 (0.73, 0.85) <0.0001
GA_samp 0.99 (0.97, 1.02) 0.52

38.3.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Serum folate concentration (nM) and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 73.31015 15.28609 53.39785 48.25736 111.3691
STOP 82.11101 13.61965 23.33618 58.27772 115.6912
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 61.75315 4.932596 118.02485 52.71864 72.33593
STOP 73.73855 3.198742 34.49795 67.51939 80.53056
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 1.883595 0.2053143 136.36224 1.518359 2.336686
STOP 1.750600 0.0952250 44.04672 1.568837 1.953422
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 27.97135 5.528478 Inf 18.98782 41.20516
STOP 17.87609 1.451797 Inf 15.24554 20.96054
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 7.533656 1.5929048 Inf 4.977703 11.402040
STOP 3.801541 0.3304497 Inf 3.206040 4.507654
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 6.036888 0.2330354 Inf 5.596997 6.511351
STOP 4.769391 0.0981154 Inf 4.580914 4.965623

38.3.4 Comparisons

Effect for every 50 unit increase in Serum folate concentration (nM).
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) Folate90.7 / Folate40.7 SCOPE 1.06 (0.95, 1.20) 0.3
PRL (ng/ml) Folate90.7 / Folate40.7 STOP 1.06 (0.95, 1.20) 0.3
hPL (μg/ml) Folate90.7 / Folate40.7 SCOPE 1.02 (0.95, 1.09) 0.6
hPL (μg/ml) Folate90.7 / Folate40.7 STOP 1.02 (0.95, 1.09) 0.6
GH2 (ng/ml) Folate90.7 / Folate40.7 SCOPE 1.00 (0.91, 1.09) 0.9
GH2 (ng/ml) Folate90.7 / Folate40.7 STOP 1.00 (0.91, 1.09) 0.9
Insulin (uU/ml) Folate90.7 / Folate40.7 SCOPE 0.90 (0.78, 1.03) 0.1
Insulin (uU/ml) Folate90.7 / Folate40.7 STOP 0.90 (0.78, 1.03) 0.1
HOMA-IR Folate90.7 / Folate40.7 SCOPE 0.86 (0.75, 1.00) 0.05
HOMA-IR Folate90.7 / Folate40.7 STOP 0.86 (0.75, 1.00) 0.05
Glucose (mmol/L) Folate90.7 / Folate40.7 SCOPE 0.95 (0.91, 0.99) 0.02
Glucose (mmol/L) Folate90.7 / Folate40.7 STOP 0.95 (0.91, 0.99) 0.02

38.3.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

38.3.6 Plots